| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.051741 |
| Chromosome: | chromosome 3 |
| Location: | 1314210 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g150500 | SNE10 | NAD-dependent epimerase/dehydratase; (1 of 1) PTHR10366//PTHR10366:SF340 - NAD DEPENDENT EPIMERASE/DEHYDRATASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTTCACCTCCACGGCGACCAACGCCACC |
| Internal bar code: | GGGGAACAGACGGGGAGGAAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 843 |
| LEAP-Seq percent confirming: | 99.5996 |
| LEAP-Seq n confirming: | 5721 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTAGCCCGATGAACCAGT |
| Suggested primer 2: | TCCTGCTAGGCTTGTTTGGT |