| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.051745 |
| Chromosome: | chromosome_17 |
| Location: | 334916 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g698365 | FAP172 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATGCGTCGTCTCCAGGTTGAGCTGCAGCTT |
| Internal bar code: | TCTCAGTGCGACGACTATATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 782 |
| LEAP-Seq percent confirming: | 99.1936 |
| LEAP-Seq n confirming: | 246 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGGCTTGGCACAATAACC |
| Suggested primer 2: | CTATTCCAGCCCTTCCTTCC |