| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.051753 |
| Chromosome: | chromosome 2 |
| Location: | 6838941 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g119350 | TMG2 | (1 of 2) K15445 - tRNA (guanine9-N1)-methyltransferase [EC:2.1.1.221] (TRMT10A, TRM10, RG9MTD2); tRNA (guanine-N1)-methyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAAGGAGGAGCTGGCACCGGGTTCGAACA |
| Internal bar code: | AATCGAGTTGGGACGAACTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 537 |
| LEAP-Seq percent confirming: | 99.4444 |
| LEAP-Seq n confirming: | 716 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTTCTTCGTGACGCCCTT |
| Suggested primer 2: | AACCTAGGCCCACCTCTTGT |