Insertion junction: LMJ.RY0402.051757_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.051757
Chromosome:chromosome 8
Location:4476710
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g382515 (1 of 1) PTHR22838//PTHR22838:SF0 - WD REPEAT PROTEIN 26-RELATED // WD REPEAT-CONTAINING PROTEIN 26 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCGAAGCTGGCCGAGGCGCGCTGCGCCT
Internal bar code:ATCGTCTCGGCACCATGGATGC

Confirmation - LEAP-Seq

LEAP-Seq distance:520
LEAP-Seq percent confirming:99.7856
LEAP-Seq n confirming:6517
LEAP-Seq n nonconfirming:14
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGCAAGGCCAAGTAAAAA
Suggested primer 2:CTGCACTCGACGGAGTAACA