| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.051765 |
| Chromosome: | chromosome 17 |
| Location: | 1420959 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g706600 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATCACCGCGTCACGCTCTACATGAAACT |
| Internal bar code: | ATGCGATGGTGAACCTCGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 915 |
| LEAP-Seq percent confirming: | 94.3089 |
| LEAP-Seq n confirming: | 116 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGCACTTGGAGATGCTTG |
| Suggested primer 2: | GCATGAGACGGAGTGCTACA |