| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.051767 |
| Chromosome: | chromosome 10 |
| Location: | 3417306 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g444500 | (1 of 7) 2.8.1.1 - Thiosulfate sulfurtransferase / Thiosulfate thiotransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATTCCACGCTGTGGTCCCATTAGCGCAC |
| Internal bar code: | AAGGATCGAGATCCGCTCGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 651 |
| LEAP-Seq percent confirming: | 99.7524 |
| LEAP-Seq n confirming: | 10474 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCATAACCCCCGATCAC |
| Suggested primer 2: | TTGCTGCTTGTACGTTTTCG |