| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.051789 |
| Chromosome: | chromosome 1 |
| Location: | 699308 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g003650 | 5'UTR_intron|intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGTTCACGCCCGCATGCACGCGCGCGCG |
| Internal bar code: | TCGCTGGGTCTGGGCCCTATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 325 |
| LEAP-Seq percent confirming: | 47.1215 |
| LEAP-Seq n confirming: | 2652 |
| LEAP-Seq n nonconfirming: | 2976 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACCAACAACAACTCACCG |
| Suggested primer 2: | GCACTAATAGCAAGCTGGGC |