Insertion junction: LMJ.RY0402.051791_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.051791
Chromosome:chromosome 12
Location:6812699
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g561550 CDS1 Mitochondrial half-size ABC transporter, membrane protein; (1 of 2) K05661 - ATP-binding cassette, subfamily B (MDR/TAP), member 6 (ABCB6) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCATGCGCACGACGACTCACCGCAATTTGC
Internal bar code:CAGTCGCAGTAAGAAGTGTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:899
LEAP-Seq percent confirming:91.766
LEAP-Seq n confirming:2541
LEAP-Seq n nonconfirming:228
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:TTGACGATGTCAGCTTCCAG
Suggested primer 2:CCTGGATCTTCTTCTCCGTG