Insertion junction: LMJ.RY0402.051807_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.051807
Chromosome:chromosome 6
Location:3003353
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g273650 (1 of 3) 1.11.2.3 - Plant seed peroxygenase / Soybean peroxygenase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGGGTAGGACTGGGGGTGCGGGGAAGCC
Internal bar code:AAGATTGCCCCGTTGCTTTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:888
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:580
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTTCTTGAGGTGGATGGC
Suggested primer 2:CCCTGGAAGACCAGTTGTGT