| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.051846 |
| Chromosome: | chromosome 16 |
| Location: | 5237761 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682600 | (1 of 1) PTHR24079//PTHR24079:SF0 - FAMILY NOT NAMED // KNIRPS-RELATED PROTEIN-RELATED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGACGTGGCTGGACACAGTACGGCGGGGCG |
| Internal bar code: | GTTGATCGGGACGGTCGATTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 837 |
| LEAP-Seq percent confirming: | 99.4188 |
| LEAP-Seq n confirming: | 2395 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAAGCAGTAGCAGGGGAC |
| Suggested primer 2: | AGTACTTTTTGTGCCCACGG |