| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.051854 |
| Chromosome: | chromosome 3 |
| Location: | 3966396 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171751 | (1 of 5) K16302 - metal transporter CNNM (CNNM) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGACACGTGTGCGTGCGCATTCGAGCTTA |
| Internal bar code: | AATCTGTCTCCAGCGTTAAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 310 |
| LEAP-Seq percent confirming: | 99.7802 |
| LEAP-Seq n confirming: | 908 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTTGTCCTTGTCCCGACC |
| Suggested primer 2: | ACTCCGTGCTCCATACCAAC |