| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.051874 |
| Chromosome: | chromosome 11 |
| Location: | 1476155 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467740 | (1 of 1) PTHR10108//PTHR10108:SF705 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGTGACACCGCTAGCCCGCCTTCGCATC |
| Internal bar code: | GTATACTATTTCTTTGCACTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 509 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 133 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGTTCATGGCAGGCTGT |
| Suggested primer 2: | GGGTTTTCATCGACCTAGCA |