| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.051878 |
| Chromosome: | chromosome 11 |
| Location: | 1910997 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g468200 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCGGAGCCGAGCTAGGGCCCACCGCAGA |
| Internal bar code: | GAATACGGGCCGCATCACACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 369 |
| LEAP-Seq percent confirming: | 99.7226 |
| LEAP-Seq n confirming: | 6470 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACGCAAACCTTATCACCG |
| Suggested primer 2: | TACGCACATCCATGACACCT |