Insertion junction: LMJ.RY0402.051884_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.051884
Chromosome:chromosome 12
Location:3160913
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g499400 CYN2,CYN18-2,CYN18B (1 of 1) K12733 - peptidyl-prolyl cis-trans isomerase-like 1 (PPIL1); Cyclophilin 18-2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGGTGCCAGGTCCCCACGCCTGCAAACTC
Internal bar code:GTGCTCCAGGTTTAAAGAGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:629
LEAP-Seq percent confirming:99.6803
LEAP-Seq n confirming:1247
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGGACAGTGCTCACACATCT
Suggested primer 2:GTTCCATCTGCAAGTCAGCA