| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.051889 |
| Chromosome: | chromosome 10 |
| Location: | 108082 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418450 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTGGCACAACTGACGAGCCCCCGAACCC |
| Internal bar code: | GACAGCAGGGCAATCCGGTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 185 |
| LEAP-Seq percent confirming: | 27.5789 |
| LEAP-Seq n confirming: | 131 |
| LEAP-Seq n nonconfirming: | 344 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTATAGGCGACTGCGGCT |
| Suggested primer 2: | TCCTTTTATCCTTCGCATGG |