| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.051893 |
| Chromosome: | chromosome 4 |
| Location: | 3885000 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g230536 | (1 of 5) 1.4.3.13 - Protein-lysine 6-oxidase / Lysyl oxidase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGAAGCGCCGGGGACGGAGGCCGGATAGA |
| Internal bar code: | TGGTCCTATCTCGCGGAGTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 402 |
| LEAP-Seq percent confirming: | 99.504 |
| LEAP-Seq n confirming: | 3009 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAATGGCAAAGAAGCACA |
| Suggested primer 2: | GTCTGTGCAGCCTTGTGTGT |