| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.051918 |
| Chromosome: | chromosome 3 |
| Location: | 8179969 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200250 | ICL3 | Putative isocitrate lyase/malate synthase; (1 of 1) 2.7.8.23 - Carboxyvinyl-carboxyphosphonate phosphorylmutase / CPEP phosphonomutase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAACGAGCTGATCGGTCTGGAGGAGAAG |
| Internal bar code: | CGCAATCAAATTCGGCATGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 393 |
| LEAP-Seq percent confirming: | 99.7067 |
| LEAP-Seq n confirming: | 9178 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGTTCTTCTCTTGCTCC |
| Suggested primer 2: | AGCACTCACGACTACATGCG |