Insertion junction: LMJ.RY0402.051942_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.051942
Chromosome:chromosome_2
Location:7866468
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre02.g141700 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):TGGGCACCTCGGCGCCTGATCAGGCCAGGG
Internal bar code:CATCACGGGTCAGGCATCGCTC

Confirmation - LEAP-Seq

LEAP-Seq distance:36
LEAP-Seq percent confirming:37.037
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:34
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGGTAGGCAGGTAGGGGC
Suggested primer 2:ACACACACACACACACACGC