| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.051950 |
| Chromosome: | chromosome 16 |
| Location: | 5835747 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677850 | (1 of 4) PF09366 - Protein of unknown function (DUF1997) (DUF1997) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGAAGCTCCTGCAGTGGCGGGAGCCACTT |
| Internal bar code: | AAGGGGCTAAAAGCAGCTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1060 |
| LEAP-Seq percent confirming: | 99.5108 |
| LEAP-Seq n confirming: | 9967 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTAACGTTGAACACCGCA |
| Suggested primer 2: | TTTGGTTTAGTTTGGGCTGG |