| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.051990 |
| Chromosome: | chromosome 17 |
| Location: | 5906538 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g740050 | (1 of 2) IPR003582//IPR011050//IPR019316 - ShKT domain // Pectin lyase fold/virulence factor // G8 domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCACCCAACCGCCCAACGGTCATTGTG |
| Internal bar code: | TCAGGCGGATGTAACTCGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 847 |
| LEAP-Seq percent confirming: | 99.7755 |
| LEAP-Seq n confirming: | 15109 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCACCTCAAACTCCCAGTA |
| Suggested primer 2: | CATGTCACGCTGAGAGGAAA |