| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.052011 |
| Chromosome: | chromosome 8 |
| Location: | 1521657 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g364850 | AC115 | putative ADP-ribosylglycosylhydrolase; (1 of 4) PTHR16222//PTHR16222:SF17 - ADP-RIBOSYLGLYCOHYDROLASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGTGCGCGTAATTTAATCAAAGGACCAG |
| Internal bar code: | TTCCGAGTACTCTGTTTTATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 860 |
| LEAP-Seq percent confirming: | 90.3922 |
| LEAP-Seq n confirming: | 461 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGCAGTAACAGGAAAGCG |
| Suggested primer 2: | GCTACAAAATGCGCAACTCA |