| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.052025 |
| Chromosome: | chromosome 16 |
| Location: | 3681237 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688700 | (1 of 22) PF01764 - Lipase (class 3) (Lipase_3) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAATGCCTGAGAGACTGACCGGCTCGGTTG |
| Internal bar code: | TACTCGTAGCGGCAGGGCATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 494 |
| LEAP-Seq percent confirming: | 98.4549 |
| LEAP-Seq n confirming: | 27654 |
| LEAP-Seq n nonconfirming: | 434 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGCAAGATGGAGTGCTTA |
| Suggested primer 2: | GATGGACACAATGCAACAGG |