| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.052035 |
| Chromosome: | chromosome 13 |
| Location: | 5154651 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g607650 | POC15 | Proteome of centriole protein 15; (1 of 1) PTHR10891//PTHR10891:SF5 - EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGATGAAGGGGCGTGTGCTCGAGCTGG |
| Internal bar code: | GCGGGTAATCCAGGCAAAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 814 |
| LEAP-Seq percent confirming: | 98.4446 |
| LEAP-Seq n confirming: | 5000 |
| LEAP-Seq n nonconfirming: | 79 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCATACGGCCAAGGATATG |
| Suggested primer 2: | TGCACTCACATCTTCTTGCC |