| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.052039 |
| Chromosome: | chromosome 8 |
| Location: | 1385604 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g364100 | (1 of 1) PF13449 - Esterase-like activity of phytase (Phytase-like) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGGCCGCCTGCTGTGTACGCCTCGGCAA |
| Internal bar code: | GCCGAAGACCGTAAGCGGGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1023 |
| LEAP-Seq percent confirming: | 99.2197 |
| LEAP-Seq n confirming: | 18056 |
| LEAP-Seq n nonconfirming: | 142 |
| LEAP-Seq n unique pos: | 105 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGACCAGCAGAGCATACA |
| Suggested primer 2: | CAGGACAACAACTTCCCGTT |