| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.052040 |
| Chromosome: | chromosome 14 |
| Location: | 3682692 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g631600 | (1 of 1) 1.5.1.40 - 8-hydroxy-5-deazaflavin:NADPH oxidoreductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTCTCGACAACCCAGACCGAACCAAAGT |
| Internal bar code: | GACTGACCTGTGACTCGCTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 810 |
| LEAP-Seq percent confirming: | 99.6793 |
| LEAP-Seq n confirming: | 5595 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAGTGGCGTTTGATTACT |
| Suggested primer 2: | CGGTATTCAGCCAACCACTT |