| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.052048 |
| Chromosome: | chromosome 13 |
| Location: | 1743881 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g574551 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGCGTAATGTTTCTGGAGGGTAATGCCAA |
| Internal bar code: | GAGCACGCTGAGGCAAGGGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 496 |
| LEAP-Seq percent confirming: | 99.3293 |
| LEAP-Seq n confirming: | 3258 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCCCAGTCCACTGCTCTTA |
| Suggested primer 2: | AACGTTACATGCGTACTGCG |