Insertion junction: LMJ.RY0402.052051_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.052051
Chromosome:chromosome 3
Location:2257168
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g157800 TRXL1,TRL6,TRX2 (1 of 1) PTHR10438:SF227 - THIOREDOXIN-LIKE 2-1, CHLOROPLASTIC-RELATED; Thioredoxin-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGCGTATTTCCCGGCAATATACCCACCG
Internal bar code:GCGCCAGACGAATCGCGGAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:942
LEAP-Seq percent confirming:99.5206
LEAP-Seq n confirming:6643
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:ATCATCCCTATCCCACCACA
Suggested primer 2:GGACGTGCTCTTCCTCAAAG