| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.052078 |
| Chromosome: | chromosome 13 |
| Location: | 2743764 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g582650 | MAPK1,LF4 | (1 of 1) K08830 - renal tumor antigen [EC:2.7.11.22] (RAGE, MOK); Mitogen-activated protein kinase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCGCCGGTTCAGCCTGTCTCGCCCGCAG |
| Internal bar code: | CAATTTCGGATGAAGGTGAGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 699 |
| LEAP-Seq percent confirming: | 99.7446 |
| LEAP-Seq n confirming: | 12109 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTTTTACGGGTCTTGGTG |
| Suggested primer 2: | CTGGGCTTTAAGCACCTCTG |