Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.052083 |
Chromosome: | chromosome_6 |
Location: | 3933662 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre06.g278187 | MOT4 | Predicted protein | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GAACTCAAGCTTGCGGCAGAGTGCTGCCGC |
Internal bar code: | GAGAACCTCAAGGAGCACGACC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 564 |
LEAP-Seq percent confirming: | 99.4194 |
LEAP-Seq n confirming: | 10616 |
LEAP-Seq n nonconfirming: | 62 |
LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAGCTAATTCCCAAACCCAA |
Suggested primer 2: | TGTTCAACAGGCCCTACACA |