| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.052085 |
| Chromosome: | chromosome 8 |
| Location: | 4060908 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g379450 | (1 of 1) K01190 - beta-galactosidase (lacZ) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGGATTTCGTGCAGCCGGGATTTGCCGC |
| Internal bar code: | GTCATTAATAGATCATACTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 492 |
| LEAP-Seq percent confirming: | 99.8195 |
| LEAP-Seq n confirming: | 3319 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGCAACGAAACAGAGGTA |
| Suggested primer 2: | AACACAATTACTCCCAGCCG |