Insertion junction: LMJ.RY0402.052099_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.052099
Chromosome:chromosome 3
Location:7214866
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g207800 ADH5,CAD1 Cinnamyl-alcohol dehydrogenase; (1 of 2) 1.1.1.195 - Cinnamyl-alcohol dehydrogenase / CAD intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTAAGGGCGGCCGGTTCTTGGGCACTCGTT
Internal bar code:GTCGTACGCCGTTCTGAAATCC

Confirmation - LEAP-Seq

LEAP-Seq distance:891
LEAP-Seq percent confirming:99.5895
LEAP-Seq n confirming:7520
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CATAACCCACGCAGTTGTTG
Suggested primer 2:TTTCCTGCAAGCCAAGAACT