| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.052137 |
| Chromosome: | chromosome 13 |
| Location: | 2786886 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g582800 | (1 of 1) 6.3.2.11 - Carnosine synthase / Carnosine synthetase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACAGAACTGAGCCTCGCTGTCTGCACACAC |
| Internal bar code: | AACGCTCGCTGGTTGGAGTAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 120 |
| LEAP-Seq percent confirming: | 79.0323 |
| LEAP-Seq n confirming: | 98 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCACTCCGTTCTCTCCTCAC |
| Suggested primer 2: | ACCAGCTACCTTCCCAACCT |