| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.052138 |
| Chromosome: | chromosome 4 |
| Location: | 1928549 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g218200 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAACATGGCGCACGCCTCCTCCGCCTCCTC |
| Internal bar code: | TGATGCCCCGGTAGCGTAACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 895 |
| LEAP-Seq percent confirming: | 95.1163 |
| LEAP-Seq n confirming: | 818 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCCCACACGCACATATAC |
| Suggested primer 2: | TTCATCCTAATGAGGTCGCC |