| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.052150 |
| Chromosome: | chromosome 16 |
| Location: | 1001733 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g649050 | CYG38 | (1 of 5) K12319 - guanylate cyclase soluble subunit beta (GUCY1B); Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGATTGCAGACGTCGAAAGCTGTCAACG |
| Internal bar code: | GAAGTGGGATGCGTGCGGGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 878 |
| LEAP-Seq percent confirming: | 98.5047 |
| LEAP-Seq n confirming: | 3162 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGTGACCACACAGATCAA |
| Suggested primer 2: | GCAGCTCAAGGTAAAGACCG |