| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.052168 |
| Chromosome: | chromosome 6 |
| Location: | 2216671 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g266250 | (1 of 15) PTHR12291:SF23 - PROTEIN MEC-15 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAATGACATGCTCCTTGTATTCCCCGTCT |
| Internal bar code: | ATTTTAGCAAATTGTAAGAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 433 |
| LEAP-Seq percent confirming: | 99.1805 |
| LEAP-Seq n confirming: | 7504 |
| LEAP-Seq n nonconfirming: | 62 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCCTTGACCTTGGACAGTA |
| Suggested primer 2: | CTTCTGCAGGGTGGTCTAGC |