| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.052185 |
| Chromosome: | chromosome_6 |
| Location: | 7017981 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g296850 | FAP81 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCTTTGCAACCCGACCACCTCCTCTCCACA |
| Internal bar code: | GTGGCGTGCCGTACCGCGGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 353 |
| LEAP-Seq percent confirming: | 99.8036 |
| LEAP-Seq n confirming: | 8131 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTCACCGTCACCTTCTGC |
| Suggested primer 2: | CGTCCGTCTCCTCTATGCTC |