| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.052185 |
| Chromosome: | chromosome_11 |
| Location: | 2448236 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g475400 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGGCGGCGGGCGTCACGGCCAGACGCACC |
| Internal bar code: | CCGCCGGCACAACGTGCCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 452 |
| LEAP-Seq percent confirming: | 95.2415 |
| LEAP-Seq n confirming: | 1321 |
| LEAP-Seq n nonconfirming: | 66 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGGACCTGGTACACTCCAG |
| Suggested primer 2: | CACAGGCCATGTACCAACTG |