| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.052186 |
| Chromosome: | chromosome 3 |
| Location: | 3605206 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g168500 | (1 of 1) PF15370 - Domain of unknown function (DUF4598) (DUF4598) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTACTGCAATCGAAGCCATGCAGAGCTGC |
| Internal bar code: | GGTGCCCGCGTGACAAGACACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 819 |
| LEAP-Seq percent confirming: | 97.1269 |
| LEAP-Seq n confirming: | 6592 |
| LEAP-Seq n nonconfirming: | 195 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACTGGGACCTCTGTGCTT |
| Suggested primer 2: | CTTGTTCATGCCTACGGGTT |