| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.052242 |
| Chromosome: | chromosome 12 |
| Location: | 1600388 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486400 | (1 of 1) PTHR22957//PTHR22957:SF26 - TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN // PROTEIN TBC-3, ISOFORM B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGCTGCTGCGCAGGATGAAGGCCTCGCT |
| Internal bar code: | GAGGTGTGCCCGTGGGTGTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 225 |
| LEAP-Seq percent confirming: | 95.9596 |
| LEAP-Seq n confirming: | 20995 |
| LEAP-Seq n nonconfirming: | 884 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATAAAGGGACTGCGTTGG |
| Suggested primer 2: | GAGGAGGGGAGAGGAGAAGA |