| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.052269 |
| Chromosome: | chromosome 3 |
| Location: | 4357774 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g175100 | (1 of 1) IPR013750//IPR016035 - GHMP kinase, C-terminal domain // Acyl transferase/acyl hydrolase/lysophospholipase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCGTAGACATGCTGCCGTATCGTGATGC |
| Internal bar code: | CCCGTACGCCGCTCGCGAGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 687 |
| LEAP-Seq percent confirming: | 95.3815 |
| LEAP-Seq n confirming: | 950 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCTGAACACACGTATGGG |
| Suggested primer 2: | ACGAAAGCAGCAGGGTCTTA |