| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.052330 |
| Chromosome: | chromosome 12 |
| Location: | 8323862 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549700 | (1 of 1) K11884 - RNA-binding protein PNO1 (PNO1, DIM2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTTACATGGTGAAAGCGAGCAGTGCCAAC |
| Internal bar code: | CGGCTCTGTAGTCGGCAGGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 294 |
| LEAP-Seq percent confirming: | 99.7627 |
| LEAP-Seq n confirming: | 841 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGCTGACTGACAACGTGC |
| Suggested primer 2: | CGCTACAGTTCTTGGCATCA |