| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.052339 |
| Chromosome: | chromosome 12 |
| Location: | 8997231 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g543850 | (1 of 1) PF09497 - Transcription mediator complex subunit Med12 (Med12) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACAAGTGGGCGCCTCATCTGCAACTCACT |
| Internal bar code: | GAAGAGGGGTGTGATGTGCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 356 |
| LEAP-Seq percent confirming: | 99.631 |
| LEAP-Seq n confirming: | 810 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATGGTACACGGCGAGATA |
| Suggested primer 2: | CCTACATGGGTGGAGCTGAT |