| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.052391 |
| Chromosome: | chromosome 8 |
| Location: | 3956960 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g378700 | (1 of 2) K13173 - arginine and glutamate-rich protein 1 (ARGLU1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGTTGTAGGGGGCGGACAGGGTTGGCGT |
| Internal bar code: | TTTACACATGAAGACTTTGATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 661 |
| LEAP-Seq percent confirming: | 99.6664 |
| LEAP-Seq n confirming: | 1195 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGATAGTCGGTGTGGAGTT |
| Suggested primer 2: | ATAGGGATAGGGACCATCGG |