| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.052394 |
| Chromosome: | chromosome 17 |
| Location: | 1534127 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g707300 | PGA3 | Phospholipid/glycerol acyltransferase; (1 of 1) PTHR23063:SF1 - 1-ACYLGLYCEROPHOSPHOCHOLINE O-ACYLTRANSFERASE 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGTGATGTTGGTCGGGCGTTGGCGGCGCG |
| Internal bar code: | GGATGATCGAGATCAGAAGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 812 |
| LEAP-Seq percent confirming: | 99.6961 |
| LEAP-Seq n confirming: | 9843 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCAACTTGGAAGAGAAAG |
| Suggested primer 2: | CGGAGTTGAGAGAGTGGAGG |