| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.052438 |
| Chromosome: | chromosome 7 |
| Location: | 4682875 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344702 | (1 of 24) IPR006553 - Leucine-rich repeat, cysteine-containing subtype | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCTACTTGCAATTGTACGACCAACCCGT |
| Internal bar code: | GCCTCGACATTGTTACCTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 133 |
| LEAP-Seq percent confirming: | 99.7406 |
| LEAP-Seq n confirming: | 1538 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTACCGGTACTGCTTC |
| Suggested primer 2: | CGGCTTTCAAGAGTTTCAGG |