| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.052438 |
| Chromosome: | chromosome 7 |
| Location: | 4682878 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g344702 | (1 of 24) IPR006553 - Leucine-rich repeat, cysteine-containing subtype | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGGTTGTTGTGCAGGCCTGCGAAGTACA |
| Internal bar code: | CCCTACCAACGGATGATGCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 163 |
| LEAP-Seq percent confirming: | 87.5184 |
| LEAP-Seq n confirming: | 596 |
| LEAP-Seq n nonconfirming: | 85 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGCTACCGGTACTGCTTC |
| Suggested primer 2: | CGGCTTTCAAGAGTTTCAGG |