| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.052448 |
| Chromosome: | chromosome 14 |
| Location: | 1753429 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g619800 | SPO11B,TOP4 | meoisis-related DNA topoisomerase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCACAACAATCGGTCCGGTAGGCCTCCGG |
| Internal bar code: | ACTGAATCCTCGTATCATTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 215 |
| LEAP-Seq percent confirming: | 96.3636 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTGCTACTGCCCCTGCTA |
| Suggested primer 2: | GGGTTGTTATGCGCTTCAAT |