| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.052454 |
| Chromosome: | chromosome 12 |
| Location: | 5175573 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g527500 | (1 of 1) K04508 - transducin (beta)-like 1 (TBL1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGCCTCCTTCGACAAGACGGTGCGGCTG |
| Internal bar code: | CGGCATACGAGGATTGTAATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 673 |
| LEAP-Seq percent confirming: | 88.6669 |
| LEAP-Seq n confirming: | 4350 |
| LEAP-Seq n nonconfirming: | 556 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTACCGTGCGTGTCATCAT |
| Suggested primer 2: | CCGGCCAGTACAATCAACTT |