Insertion junction: LMJ.RY0402.052477_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.052477
Chromosome:chromosome 2
Location:961178
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre02.g079926 PHC64 Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCACGCCAGGCGCACACTGTACTTGCTCG
Internal bar code:TATGCATGCCCTCGGGCACTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:514
LEAP-Seq percent confirming:99.4077
LEAP-Seq n confirming:1007
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:TTTCCTAGACTGCCACGCTT
Suggested primer 2:TGTGAAGTTGTTGTAGCCGC