| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.052484 |
| Chromosome: | chromosome 7 |
| Location: | 610908 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g316850 | MCM4 | (1 of 1) K02212 - DNA replication licensing factor MCM4 (MCM4, CDC54); Minichromosome maintenance protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTCACTCCGCTGGCGAGGGTGGGCGGT |
| Internal bar code: | CCTGTCCCCGCGGTCTGGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 89 |
| LEAP-Seq percent confirming: | 99.0446 |
| LEAP-Seq n confirming: | 311 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTAGCCGTTCATGTGGAGT |
| Suggested primer 2: | CAGTGGTTTGGTGTGGTGAG |